110 http://isa.epfl.ch/imoniteur_ISAP/ itffichecours.txt?path=EPFL.Etu.FicheCours.HeaderFR itffichecours.txt?path=EPFL.Etu.FicheCours.GlobalNav_js fr 2026 84482813 2936295 84482813 2305107546 2305107546 0 1 Mineurs MINEUR !itffichecours.htm?ww_i_matiere=1942238872&ww_x_anneeAcad=2305107546&ww_i_section=84482813&ww_i_niveau=2936295 1696552884 1696552963 2024-2025 COURSE BOOKLET CH-319 Experimental biochemistry and biophysics Experimental biochemistry and biophysics Lecturers Hovius Ruud Language of teaching English Cursus Biotechnology minor(2024-2025, Mineur : Biotechnologie 2024-25)
Chemistry(2024-2025, CGC BA orientation chimie 2024-25)
SemesterMand.OptionFilières
BA6x
Ex
 Credits 4 Withdrawal Unauthorized It is not allowed to withdraw from this subject after the registration deadline. Session Summer Workload 120h Exam During the semester Number of positions Il n'est pas autorisé de se retirer de cette matière après le délai d'inscription. Semester Spring Weeks 14 Hours 6 weekly
TP 6 weekly
Study planSemesterMand.OptionFilières
CGC Orientation 2024-25BA6x
Mineur : Biotechnologie 2024-25Ex
Cursus Sem. Type
Biotechnology minor E Opt.
Chemistry BA6 Obl.
Summary A 7-week long (4+8 h) experiment where you plan and construct a fluorescent sensor protein starting from DNA bricks. The protein will be expressed in and purified from E.coli, characterized by biochemical and spectroscopic methods. Your report will be in the style of a scientific paper or poster Content

i) Molecular biology: DNA fragment isolation by PCR, DNA restriction and ligation, plasmid purification and sequence analysis, agarose gel electrophoresis.

 

ii) Biochemistry: Bacterial protein expression, protein purification by affinity chromatography, analysis by SDS-PAGE and spectroscopy, fluorescent labelling

 

iii) Biophysics: Fluorescence spectroscopy, enzyme kinetics or molecular interactions.

 Keywords

Molecular biology - cloning

 

Protein expression & purification

 

Bio-chemical and bio-physical chracterisation

 

Sensor proteins

 

Reporting

 Learning Prerequisites Required courses

Admission to the TP is conditional on the successful completion of 2 of the 3 courses: CH-210 Biochimie, CH-313 Chemical biology, CH-311 Macromolecular structure and interactions

 

 Recommended courses

Molecular and cellular biophysics I (CH-311)

Chemical Biology (CH-313)

 Important concepts to start the course

genetic engineering & DNA manipulation; protein synthesis; DNA & protein analysis; absorbance and fluorescence spectrometry; enzymology, molecular interactions

 Learning Outcomes
  • Design cloning strategy
  • Produce a scientific report and high-quality lab journal
  • Integrate Good laboratory behavior and wet lab practice
  • Assess / Evaluate your data critically
  • Produce a purified expressed protein
  • Analyze proteins and DNA
  • Characterize sensor function
  • Use common sense and logical deduction
  • Interpret protein structure data
  • Design cloning strategy
  • Produce a scientific report and high-quality lab journal
  • Integrate Good laboratory behavior and wet lab practice
  • Assess / Evaluate Evaluate your data critically
  • Produce a purified expressed protein
  • Analyze proteins and DNA
  • Characterize sensor function
  • Interpret protein structure data
 Transversal skills
  • Collect data.
  • Continue to work through difficulties or initial failure to find optimal solutions.
  • Write a scientific or technical report.
  • Plan and carry out activities in a way which makes optimal use of available time and other resources.
 Teaching methods

Students prepare and discuss experimental approach

 

Laboratory experimentation

 

Discussion of experimental progress and results

 

Reporting on scientific level

Topiocal presentations

 Expected student activities

Good theoretical preparation & planning of lab work before doing experiments; skillful execution of experiments, being organized & keeping a lab book; thorough analysis of results, writting a scientific-grade report; respecting security rules and fellow students.

 Assessment methods

valuation of preparation and planning

 

Evaluation of experimental execution, including good laboratory behaviour

 

Evaluation of comprehension through discussion & written questions

 

Evaluation of report: including structure, data treatment and presentation, critical attitude , comparison to scientific literature

 


 Supervision Office hours Yes Assistants Yes Others

Discussions are possible at office hours, depending on the availability of assistants and lecturer,

Experimentation might be possible at office hours, depending on the availability of assistants and lecturer, and only upon the allowance of lecturer.

 

 Resources Bibliography

Bibliography

 

TP manual

 

Biochemistry & Biophysics text books

 

ApE; a plasmid editor free software

 

Methods, Structures, and other useful info via Moodle

 Notes/Handbook

Manual

 Websites Moodle Link Prerequisite for

big plus for Msc courses and for semester or diploma projects in chemical biology or biophysics

 


 Chimie, 2024-2025, Bachelor semestre 6 Chemistry, 2024-2025, Bachelor semester 6 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Bachelor semestre 6 BA6 ETE Bachelor semester 6 BA6 Chimie CGC_OR_CHIM Chemistry CGC_OR_CHIM
Chimie et génie chimique CGC Chemistry and Chemical Engineering CGC
obligatoire mandatory
6 TP TP Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Hovius Rudolf 105423
Chimie et génie chimique CGC Chemistry and Chemical Engineering CGC
1942238872 Experimental biochemistry and biophysics Experimental biochemistry and biophysics 4
Pendant le semestre During the semester Crédit Credits 4 Session été Session summer ETE ETE
Mineur en Biotechnologie, 2024-2025, Semestre printemps Biotechnology minor, 2024-2025, Spring semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre printemps E ETE Spring semester E Mineur en Biotechnologie MIN_BIOTECH Biotechnology minor MIN_BIOTECH
optionnel optional
6 TP TP Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Hovius Rudolf 105423
Chimie et génie chimique CGC Chemistry and Chemical Engineering CGC
1942238872 Experimental biochemistry and biophysics Experimental biochemistry and biophysics 4
Pendant le semestre During the semester Crédit Credits 4 Session été Session summer ETE ETE
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