110 http://isa.epfl.ch/imoniteur_ISAP/ itffichecours.txt?path=EPFL.Etu.FicheCours.HeaderFR itffichecours.txt?path=EPFL.Etu.FicheCours.GlobalNav_js fr 2026 945244 6683147 945244 2305107546 2305107546 0 0 Microtechnique MT STI Faculté des sciences et techniques de l'ingénieur School of Engineering !itffichecours.htm?ww_i_matiere=47830388&ww_x_anneeAcad=2305107546&ww_i_section=945244&ww_i_niveau=6683147 1696552884 1696552963 2024-2025 COURSE BOOKLET MICRO-561 Biomicroscopy I Biomicroscopy I Lecturers Altug Hatice Language of teaching English Cursus Biomedical technologies minor(2024-2025, Mineur : Technologies biomédicales 2024-25)
Computational and Quantitative Biology (edoc)(2024-2025, EDCB 2024-25)
Life Sciences Engineering(2024-2025, SV - Master 2023)
Life Sciences Engineering(2024-2025, SV - Master 2024)
Microengineering(2024-2025, MT - Master 2023)
Microengineering(2024-2025, MT - Master 2024)
Minor in Imaging(2024-2025, Mineur : Imaging 2024-25)
Minor in life sciences engineering (2024-2025, Mineur : Sciences et technologies du vivant 2024-25)
Neuro-X minor(2024-2025, Mineur : Neuro-X 2024-25)
Neuro-X(2024-2025, SNX - Master 2023)
Neuro-X(2024-2025, SNX - Master 2024)
Photonics (edoc)(2024-2025, EDPO 2024-25)
Photonics minor(2024-2025, Photonics 2024-25)
SemesterMand.OptionFilières
x
Hx
MA1, MA3x
MA1, MA3xA
MA1, MA3xB L
 Credits 4 Session Winter Workload 120h Exam Written Number of positions Semester Fall Weeks 14 Hours 4 weekly
Courses 2 weekly
Exercises 2 weekly
Study planSemesterMand.OptionFilières
EDCB - Biologie computationnelle et quantitative 2024-25x
EDPO - Photonics 2024-25x
Ingénierie des sciences du vivant 2024-25MA1xB L
Ingénierie des sciences du vivant 2024-25MA3xB L
Microtechnique 2024-25MA1xA
Microtechnique 2024-25MA3xA
Mineur : Imaging 2024-25Hx
Mineur : Ingénierie des Sciences du vivant 2024-25Hx
Mineur : Neuro-X 2024-25Hx
Mineur : Technologies biomédicales 2024-25Hx
Minor : Photonics 2024-25Hx
Neuro-X 2024-25MA1x
Neuro-X 2024-25MA3x
Cursus Sem. Type
Biomedical technologies minor H Opt.
Computational and Quantitative Biology Opt.
Life Sciences Engineering MA1, MA3 Opt.
Microtechnics MA1, MA3 Opt.
Minor in Imaging H Opt.
Minor in life sciences engineering H Opt.
Neuro-X minor H Opt.
Neuro-X MA1, MA3 Opt.
Photonics minor H Opt.
Photonics Opt.
Summary Introduction to geometrical and wave optics for understanding the principles of optical microscopes, their advantages and limitations. Describing the basic microscopy components and the commonly used biomicrocopy methods such as widefield and fluorescence. Content

Geometrical and matrix (ABCD) optics, wave and Fourier optics, point-spread function (PSF), resolution and contrast, microscope elements (objectivs, eyepiece, filters, illuminations, detectors), fluorescence microscopy, and preparation of biological samples for microscopy.

 Keywords

Optical microscopy, fluorescence, wide field microscopy.

 Learning Prerequisites Required courses

Analysis IV, Linear algebra, General physics III/IV.

 Important concepts to start the course

Basic matrix calculations, Fourier transformation, electromagnetic waves, refraction and reflection.

 Learning Outcomes By the end of the course, the student must be able to:
  • Sketch basic optical systems.
  • Sketch wide field and fluorescence microscopes.
  • Estimate the resolution of imaging systems.
  • Propose a suitable microscopy configuration for imaging a sample.
  • Characterize the basic elements of a microscope
  • Sketch basic optical systems.
  • Sketch wide field and fluorescence microscopes.
  • Estimate the resolution of imaging systems.
  • Propose a suitable microscopy configuration for imaging a sample.
  • Characterize the basic elements of a microscope
 Transversal skills
  • Communicate effectively with professionals from other disciplines.
 Teaching methods

Lecturing with exercises.

 Expected student activities

Following the lecturing and solving the exercises regularly is necessary for mastering the course contents. The solutions of the exercises are distributed at the next lecture. The student is invited to find his/her own solutions and to discuss them with the assistants.

 Assessment methods

Written exam during the winter exam session

 Supervision Office hours No Assistants Yes Forum Yes Others Possible to take dates. Resources Bibliography

¿ Fundamentals of Light Microscopy and Electronic Imaging, 2nd Edition, by Murphy and Davidson. Wiley-Blackwell (2013).

¿ Fundamentals of Photonics, 2nd Edition, by Saleh and Teich. Wiley (2007).

¿ Geometrical and matrix optics: José-Philippe Pérez, Optique: fondements et applications (2004).

¿ Eugene Hecht, Optics (2002).

¿ Miles V. Klein and Thomas E. Furtak, Optics (1986).

 Ressources en bibliothèque
  • Optique : fondements et applications / Pérezhttp://library.epfl.ch/en/beast?isbn=9782100484973
  • Optics / Hechthttp://library.epfl.ch/en/beast?isbn=9781292096933
  • Optics / Kleinhttp://library.epfl.ch/en/beast?isbn=0471872970
  • Principles of optics: electromagnetic theory of propagation, interference and diffraction of light / Bornhttp://library.epfl.ch/en/beast?isbn=0-521-64222-1
  • Fundamentals of Light Microscopy and Electronic Imaging / Murphyhttp://library.epfl.ch/en/beast?isbn=0-471-25391-X
  • Fundamentals of Photonics / Salehhttp://library.epfl.ch/en/beast?isbn=978-0-471-35832-9
 Notes/Handbook

Script covering geometrical and matrix optics, Fourier optics, microscopy and fluorescence. Script chapters and course slides are published on Moodle.

 Websites Moodle Link Prerequisite for

Biomicroscopy II

 Microtechnique, 2024-2025, Master semestre 1 Microengineering, 2024-2025, Master semester 1 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 1 MA1 HIVER Master semester 1 MA1 Microtechnique MT Microengineering MT
Microtechnique MT Microengineering MT
A optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Microtechnique, 2024-2025, Master semestre 3 Microengineering, 2024-2025, Master semester 3 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 3 MA3 HIVER Master semester 3 MA3 Microtechnique MT Microengineering MT
Microtechnique MT Microengineering MT
A optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Ingénierie des sciences du vivant, 2024-2025, Master semestre 1 Life Sciences Engineering, 2024-2025, Master semester 1 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 1 MA1 HIVER Master semester 1 MA1 Ingénierie des sciences du vivant SV Life Sciences Engineering SV
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
B L optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Ingénierie des sciences du vivant, 2024-2025, Master semestre 3 Life Sciences Engineering, 2024-2025, Master semester 3 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 3 MA3 HIVER Master semester 3 MA3 Ingénierie des sciences du vivant SV Life Sciences Engineering SV
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
B L optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Neuro-X, 2024-2025, Master semestre 1 Neuro-X, 2024-2025, Master semester 1 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 1 MA1 HIVER Master semester 1 MA1 Neuro-X NX Neuro-X NX
Neuro-X NX Neuro-X NX
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Neuro-X, 2024-2025, Master semestre 3 Neuro-X, 2024-2025, Master semester 3 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Master semestre 3 MA3 HIVER Master semester 3 MA3 Neuro-X NX Neuro-X NX
Neuro-X NX Neuro-X NX
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Biologie computationnelle et quantitative (edoc), 2024-2025 Computational and Quantitative Biology (edoc), 2024-2025 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Biologie computationnelle et quantitative (edoc) EDCB Computational and Quantitative Biology (edoc) EDCB
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Mineur en Imaging, 2024-2025, Semestre automne Minor in Imaging, 2024-2025, Autumn semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre automne H HIVER Autumn semester H Mineur en Imaging MIN_IMG Minor in Imaging MIN_IMG
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Mineur en Ingénierie des Sciences du Vivant, 2024-2025, Semestre automne Minor in life sciences engineering , 2024-2025, Autumn semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre automne H HIVER Autumn semester H Mineur en Ingénierie des Sciences du Vivant MIN_SV Minor in life sciences engineering MIN_SV
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Mineur en Neuro-X, 2024-2025, Semestre automne Neuro-X minor, 2024-2025, Autumn semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre automne H HIVER Autumn semester H Mineur en Neuro-X MIN_NX Neuro-X minor MIN_NX
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Mineur en Technologies biomédicales, 2024-2025, Semestre automne Biomedical technologies minor, 2024-2025, Autumn semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre automne H HIVER Autumn semester H Mineur en Technologies biomédicales MIN_BIOMED Biomedical technologies minor MIN_BIOMED
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Photonics minor, 2024-2025, Semestre automne Photonics minor, 2024-2025, Autumn semester Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Semestre automne H HIVER Autumn semester H Photonics minor MIN_PHOTONICS Photonics minor MIN_PHOTONICS
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
Photonique (edoc), 2024-2025 Photonics (edoc), 2024-2025 Ecole polytechnique fédérale de Lausanne EPFL Ecole polytechnique fédérale de Lausanne EPFL 2024-2025 Photonique (edoc) EDPO Photonics (edoc) EDPO
optionnel optional
2 Cours Courses Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
2 Exercices Exercises Heure(s) hebdo x 14 semaines Hour(s) per week x 14 weeks H hebdo H hebdo Altug Hatice 231181
Ingénierie des sciences du vivant SV Life Sciences Engineering SV
47830388 Biomicroscopy I Biomicroscopy I 4
Ecrit Written Crédit Credits 4 Session hiver Session winter HIV HIV
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